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Lunam T-40i application for force sensing with geometrically anisotropic probes

“Optical Force Sensing With Cylindical Microcontainers”

Recently, the paper: “Optical Force Sensing With Cylindical Microcontainers” has been published in the Particle & Particle System Characterization Journal.

In this work Professor Denz and her team: Mr. Meissner and Dr. Oliver, use our force measurement system commercially available:  Lunam T-40i, to measure forces on cylindrical probes (*) in order to establish whether the established methodology for force sensing based on spherical objects still applies for other geometrically anisotropic probes. read more…

HORIZONTE PYME CALL

The Agencia Estatal de Investigación has awarded IMPETUX with a grant from the HORIZONTE PYME call. We are very grateful for this recognition!!.

Influence of experimental parameters on the laser heating of an optical trap

An interesting new article have been published today in Scientific Reports related with IMPETUX technology:

Influence of experimental parameters on the laser heating of an optical trap – Català, F. et al.

In optical tweezers, heating of the sample due to absorption of the laser light is a major concern as temperature plays an important role at microscopic scale deeply dicused in this article.

read more…

Extending calibration-free force measurements to optically-trapped rod-shaped samples

An interesting new article have been published today in Scientific Reports related with IMPETUX technology:

Extending calibration-free force measurements to optically-trapped rod-shaped samples – Català, F. et al.

In this article the authors demonstrate that the beam momentum detection can be applied to measure forces on optically trapped cylinders, without the need for previous trap calibration or complete understanding of the trapping dynamics. read more…

Japan Market Expansion Competition Award

logo_jmec

The past 7th of June, The Japan Market Expansion Competition (JMEC) announced the winners of the JMEC 22 program at its annual JMEC Awards Ceremony at the Tokyo American Club.

We are very glad and proud to say that “Team 7″, 3rd Prize winners of JMEC 22, worked on our project !!! read more…

Events

IMPETUX will be at Optical Trapping Applications

Pinnacle Vancouver Harbourfront Hotel, Vancouver, Canada

Arnau Farré is an invited speaker with the talk: Force Measurements in Complex Samples with Optical Tweezers and we will be showing our demo at the exhibition.

Meet us at tbooth 106!!

Find out more information about the event at OSA website.

Key Bibliography

Here you will find useful material published related with our technology and products

Papers

Optical trapping has become an optimal choice for biological research at the microscale due to its noninvasiveperformance and accessibility for quantitative studies, especially on the forces involved inbiological processes. However, reliable force measurements depend on the calibration of the opticaltraps, which is different for each experiment and hence requires high control of the local variables,especially of the trapped object geometry. Many biological samples have an elongated, rod-likeshape, such as chromosomes, intracellular organelles (e.g., peroxisomes), membrane tubules, certainmicroalgae, and a wide variety of bacteria and parasites. This type of samples often requires severaloptical traps to stabilize and orient them in the correct spatial direction, making it more difficult todetermine the total force applied. Here, we manipulate glass microcylinders with holographic opticaltweezers and show the accurate measurement of drag forces by calibration-free direct detection ofbeam momentum.

Measuring forces inside living cells is still a challenge due the characteristics of the trapped organelles (non-spherical, unknown size and index of refraction) and the cell cytoplasm surrounding them heterogeneous and dynamic, non-purely viscous). Here, we show how two very recent methods overcome these limitations: on the one hand, forces can be measured in such environment by the direct detection of changes in the light momentum; on the other hand, an active-passive calibration technique provides both the stiffness of the optical trap as well as the local viscoelastic properties of the cell cytoplasm.

  • Martín-Badosa, F. Català, J. Mas, M. Montes-Usategui, A. Farré, F. Marsà. “Force measurement in the manipulation of complex samples with holographic optical tweezers” 15th workshop on Information Optics (WIO), 2016.
  • Derek Craig, Alison McDonald, Michael Mazilu, Helen Rendall, Frank Gunn-Moore, and Kishan Dholakia. “ Enhanced Optical Manipulation of Cells Using Antireflection Coated Microparticles”.ACS Photonics, 2 (10), pp 1403–1409, (2015).

    In molecular studies, an optically trapped bead may be functionalized to attach to a specific molecule, whereas in cell studies, direct manipulation with the optical field is usually employed. Using this approach, several methods may be used to measure forces with an optical trap. However, each has its limitations and requires an accurate knowledge of the sample parameters.6,7 In particular, force measurements can be challenging when working with nonspherical particles or in environments with an inhomogeneous viscosity, such as inside the cell. Recent developments in the field are moving toward obtaining direct force measurements by detecting light momentum changes. For this approach, the calibration factor only comes from the detection instrumentation and negates the requirement to recalibrate for changes in experimental conditions”.

  • Xing Ma, Anita Jannasch, Urban-Raphael Albrecht, Kersten Hahn, Albert Miguel-López, Erik Schäffer, and Samuel Sánchez. “Enzyme-Powered Hollow Mesoporous Janus Nanomotors”. Nano Lett., 15 (10), pp 7043–7050, (2015).

    “Using optical tweezers, we directly measured a holding force of 64 ± 16 fN, which was necessary to counteract the effective self-propulsion force generated by a single nanomotor. The successful demonstration of biocompatible enzyme-powered active nanomotors using biologically benign fuels has a great potential for future biomedical applications.”

 In this work, the authors show the feasibility of combining optical tweezers (single-beam gradient traps) with the determination of forces using the measurement of the light momentum change.

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