Comunication

News

Extending calibration-free force measurements to optically-trapped rod-shaped samples

An interesting new article have been published today in Scientific Reports related with IMPETUX technology:

Extending calibration-free force measurements to optically-trapped rod-shaped samples – Català, F. et al.

In this article the authors demonstrate that the beam momentum detection can be applied to measure forces on optically trapped cylinders, without the need for previous trap calibration or complete understanding of the trapping dynamics. Since Force measurement based on beam momentum detection is independent of any local parameter present in the experiment, in addition to rod-shaped samples, the method described and based on IMPETUX patented Technology can be undertaken in non-viscous media, such an interior of a Cell. read more…

Japan Market Expansion Competition Award

logo_jmec

The past 7th of June, The Japan Market Expansion Competition (JMEC) announced the winners of the JMEC 22 program at its annual JMEC Awards Ceremony at the Tokyo American Club.

We are very glad and proud to say that “Team 7″, 3rd Prize winners of JMEC 22, worked on our project !!! read more…

Impetux: an innovative SME

pyme_innovadora_mineco-EN_web-2018

Impetux has recently received the Innovative SME certification. This certification is awarded by the Spanish Economics and Competitiveness Ministry in recognition of the investment done in research.

It’s time to test new technology!

IMPETUX wants to start the new year offering new services.

A new free loan service is now available for anyone who wants to test our optical force measurement systems. If you have your own optical tweezers and you are working on some experiments where you need to measure the forces applied while manipulating, these are your instruments.

read more…

IMPETUX at CLUB21

Yesterday we participated in the RN4 radio program ‘Club 21’, where we talked about our vision, our mission and our values and gave an overview about the microscopic hands made of light that are the optical tweezers. You can hear the whole program here. (in catalan)

 

IMPETUX method

Here we have two more recent articles talking about our mehod to measure optical forces:

Derek Craig, Alison McDonald, Michael Mazilu, Helen Rendall, Frank Gunn-Moore, and Kishan Dholakia. “ Enhanced Optical Manipulation of Cells Using Antireflection Coated Microparticles”.ACS Photonics, 2 (10), pp 1403–1409, (2015)

“In molecular studies, an optically trapped bead may be functionalized to attach to a specific molecule, whereas in cell studies, direct manipulation with the optical field is usually employed. Using this approach, several methods may be used to measure forces with an optical trap. read more…

Events

IMPETUX will be at Optical Trapping Applications

Pinnacle Vancouver Harbourfront Hotel, Vancouver, Canada

Arnau Farré is an invited speaker with the talk: Force Measurements in Complex Samples with Optical Tweezers and we will be showing our demo at the exhibition.

Meet us at tbooth 106!!

Find out more information about the event at OSA website.

Key Bibliography

Here you will find useful material published related with our technology and products

Papers

Optical trapping has become an optimal choice for biological research at the microscale due to its noninvasiveperformance and accessibility for quantitative studies, especially on the forces involved inbiological processes. However, reliable force measurements depend on the calibration of the opticaltraps, which is different for each experiment and hence requires high control of the local variables,especially of the trapped object geometry. Many biological samples have an elongated, rod-likeshape, such as chromosomes, intracellular organelles (e.g., peroxisomes), membrane tubules, certainmicroalgae, and a wide variety of bacteria and parasites. This type of samples often requires severaloptical traps to stabilize and orient them in the correct spatial direction, making it more difficult todetermine the total force applied. Here, we manipulate glass microcylinders with holographic opticaltweezers and show the accurate measurement of drag forces by calibration-free direct detection ofbeam momentum.

Measuring forces inside living cells is still a challenge due the characteristics of the trapped organelles (non-spherical, unknown size and index of refraction) and the cell cytoplasm surrounding them heterogeneous and dynamic, non-purely viscous). Here, we show how two very recent methods overcome these limitations: on the one hand, forces can be measured in such environment by the direct detection of changes in the light momentum; on the other hand, an active-passive calibration technique provides both the stiffness of the optical trap as well as the local viscoelastic properties of the cell cytoplasm.

  • Martín-Badosa, F. Català, J. Mas, M. Montes-Usategui, A. Farré, F. Marsà. “Force measurement in the manipulation of complex samples with holographic optical tweezers” 15th workshop on Information Optics (WIO), 2016.
  • Derek Craig, Alison McDonald, Michael Mazilu, Helen Rendall, Frank Gunn-Moore, and Kishan Dholakia. “ Enhanced Optical Manipulation of Cells Using Antireflection Coated Microparticles”.ACS Photonics, 2 (10), pp 1403–1409, (2015).

    In molecular studies, an optically trapped bead may be functionalized to attach to a specific molecule, whereas in cell studies, direct manipulation with the optical field is usually employed. Using this approach, several methods may be used to measure forces with an optical trap. However, each has its limitations and requires an accurate knowledge of the sample parameters.6,7 In particular, force measurements can be challenging when working with nonspherical particles or in environments with an inhomogeneous viscosity, such as inside the cell. Recent developments in the field are moving toward obtaining direct force measurements by detecting light momentum changes. For this approach, the calibration factor only comes from the detection instrumentation and negates the requirement to recalibrate for changes in experimental conditions”.

  • Xing Ma, Anita Jannasch, Urban-Raphael Albrecht, Kersten Hahn, Albert Miguel-López, Erik Schäffer, and Samuel Sánchez. “Enzyme-Powered Hollow Mesoporous Janus Nanomotors”. Nano Lett., 15 (10), pp 7043–7050, (2015).

    “Using optical tweezers, we directly measured a holding force of 64 ± 16 fN, which was necessary to counteract the effective self-propulsion force generated by a single nanomotor. The successful demonstration of biocompatible enzyme-powered active nanomotors using biologically benign fuels has a great potential for future biomedical applications.”

 In this work, the authors show the feasibility of combining optical tweezers (single-beam gradient traps) with the determination of forces using the measurement of the light momentum change.

By continuing to use the site, you agree to the use of cookies. more information

The cookie settings on this website are set to "allow cookies" to give you the best browsing experience possible. If you continue to use this website without changing your cookie settings or you click "Accept" below then you are consenting to this.

Close