SENSOCELL™ – Optical tweezers platform
Multiple optical trapping and simultaneous force measurements.
Our optical tweezers platform SENSOCELL™ brings you the possibility to explore motor proteins interactions in the very inside of living cells. Do not limit yourself to in vitro experiments!
- Trap native structures of the cell like vesicles or organelles or use functionalized beads.
- Measure stall forces of motor proteins inside living cells.
- Measure parallel and perpendicular to the filament components of the force exerted on the moving cargo.
- Multiple optical trapping and simultaneous force measurements allow studying the cooperation and competition processes of various molecular motors.
Measurement of the stall force of kinesins in living cells.
In collaboration with the University of Barcelona.
Motor proteins are responsible for different fundamental biological processes inside cells. One of these functions, of vital importance for the cell survival, is the intracellular transport of vesicles and organelles. Kinesin is the microtubule-based protein that performs the plus-end-directed motion. The protein generates the mechanical work required to move cargos, by means of the hydrolysis of ATP molecules. In cells, lipid droplets can be used as targets for trapping and analysis of the force of the motor proteins propelling them. Here we show the measurement of the stall force of one of these lipid droplets in an A549 cell.
Measurement of forces inside cells allows exploring the rich interplay between multiple motor proteins simultaneously pulling on the same vesicle/organelle. Here we show an example illustrating two opposed scenarios: cooperation and competition. Measurements like those shown here are an example of the potential of SENSOCELL™ in the motor protein field, which offers the possibility of measuring forces in traditionally difficult or impossible experiments.