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Microrheology of soft biological samples using SENSOCELL optical tweezers.

Microrheology of soft biological samples using SENSOCELL optical tweezers.

Microrheology of soft biological samples using SENSOCELL optical tweezers.

 

It is already available an application note where we show how SENSOCELL optical tweezers can be used to measure the viscoelastic properties of soft hydrogels, extracellular matrices or living cells, with stiffnesses ranging from tens of Pa to several kPa and at probing frequencies up to the kHz regime.

The distinctive technology used by SENSOCELL allows carrying out mechanical characterization inside soft samples in a seamless, quick and calibration-free manner.

Have a look a it at the ACTICE Microrheology section on our website here.

Our download now the application note:

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Fig. 1

Time dependence of trap position (top), force signal (middle) and Ca2+ fluorescence signal (bottom) recorded during a membrane tether pulling experiment. The force and Ca2+ ion channel activity signals exhibit negative correlation.

Fig. 2

Force and displacement data for two beads stimulated at frequencies of 4 Hz and 0.25 Hz. The progressive reduction of the beads displacement is in concomitancy with an increase of the applied force and loading rate.

Fig. 1

Schematic representation of the optical tweezers experiment. A fibronectin-coated bead is trapped, brought into contact with the cell membrane and stimulated with oscillations at different frequencies.

Fig.2

Time dependence of trap 1 and trap 2 position (top) and force (bottom) data along the different steps of the described dual tether pulling experiment.

Fig.1

Confocal video of the described dual tether pulling assay performed on a neuron axon. Courtesy of M. Krieg lab (ICFO).

Fig. 1

Time dependence of trap position (top), force signal (middle) and Ca2+ fluorescence signal (bottom) recorded during a membrane tether pulling experiment. The force and Ca2+ ion channel activity signals exhibit negative correlation.

Video 1

Confocal video showing a DVA neuron Ca2+ ion channel activity in a dynamic optical trapping assay. The pulling rate applied to the membrane tether is progressively increased. Scale bar = 5µm. Acquired at 10Hz.