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Rheological measurements to understand Cell-ECM interactions with SENSOCELL

Rheological measurements to understand Cell-ECM interactions with SENSOCELL

Last week  we could read a fascinating project story related with one of our customers.

The project, led by Pere Roca-Cusachs at IBEC and entitled: Mechano·Control: mechanical control of biological function, aims to understand and control cellular mechanics from molecules to organs.

The project team is already achieving important goals and performing amazing experiments.

For example, they have been doing rheological measurements to understand Cell-ECM interactions and see how they can alter the mechanical properties of the cell cytoeskeleton with SENSOCELL.

The finding will help fight breast cancer.

For more details, have a look at the interesting interview with Zanetta Kechgia, a postdoc of the team led by Dr Pere Roca-Cusachs at IBEC here.

 

📌If you are interested in how our optical tweezers system: SENSOCELL can be used for cell micro-rheology measurements, cell nucleus indentation experiments, neuron mechanosensation studies, among others applications,  do not hesitate to have a look at the application section or directly contact us.

More news:

Fig. 1

Time dependence of trap position (top), force signal (middle) and Ca2+ fluorescence signal (bottom) recorded during a membrane tether pulling experiment. The force and Ca2+ ion channel activity signals exhibit negative correlation.

Fig. 2

Force and displacement data for two beads stimulated at frequencies of 4 Hz and 0.25 Hz. The progressive reduction of the beads displacement is in concomitancy with an increase of the applied force and loading rate.

Fig. 1

Schematic representation of the optical tweezers experiment. A fibronectin-coated bead is trapped, brought into contact with the cell membrane and stimulated with oscillations at different frequencies.

Fig.2

Time dependence of trap 1 and trap 2 position (top) and force (bottom) data along the different steps of the described dual tether pulling experiment.

Fig.1

Confocal video of the described dual tether pulling assay performed on a neuron axon. Courtesy of M. Krieg lab (ICFO).

Fig. 1

Time dependence of trap position (top), force signal (middle) and Ca2+ fluorescence signal (bottom) recorded during a membrane tether pulling experiment. The force and Ca2+ ion channel activity signals exhibit negative correlation.

Video 1

Confocal video showing a DVA neuron Ca2+ ion channel activity in a dynamic optical trapping assay. The pulling rate applied to the membrane tether is progressively increased. Scale bar = 5µm. Acquired at 10Hz.