News

Rheological measurements to understand Cell-ECM interactions with SENSOCELL

Rheological measurements to understand Cell-ECM interactions with SENSOCELL

An interstinAn intersting project story: the team led by Dr Pere Roca-Cusachs at IBEC is already achieving important goals and performing amazing experiments. They have been doing rheological measurements to understand Cell-ECM interactions and see how they can alter the mechanical properties of the cell cytoeskeleton with SENSOCELL.

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EMBO | EMBL Symposium : Life at the Periphery

EMBO | EMBL Symposium : Life at the Periphery

The first event of this 2021 in which IMPETUX will be present will be virtual and will be the EMBO|EMBL Symposium.: Life at the Periphery: Mechanobiology of the Cell Surface.
We will be participating with a talk and a virtual booth.  Registration deadline: 8 Feb 2021

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Events

EMBO | EMBL Symposium : Life at the Periphery

The first event of this 2021 in which IMPETUX will be present will be virtual and will be the EMBO|EMBL Symposium.: Life at the Periphery: Mechanobiology of the Cell Surface.
We will be participating with a talk and a virtual booth.  Registration deadline: 8 Feb 2021

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CONFERENCES: Nanoengineering for Mechanobiology

IMPETUX will be present at the Nanoengineering for Mechanobiology symposium, hold on from November 30 to December 3, 2020  on-line.

We will be participating with an interesting talk on Monday the 30th of November

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Cytoskeleton Day in Paris

IMPETUX will be present at the Cytoskeleton Day,  hold on November  10, 2020  in Paris. We will be sharing interesting data acquired with SENSOCELL, the unique optical tweezers platform for mechanobiology, with a short talk at 11:15 h If you are interested in our latest developments an applications of SENSOCELL, do not hesitate to join us! Where → PARIS CYTOSKELETON DAY: A meet held entirely online When → Tuesday, November 10, 2020, at 11:15h How → Register here   More news:

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Scientific Publications

Here you will find useful material published related with IMPETUX’s technology and products.

 

Publications from IMPETUX’s customers:

 

In this work, the authors use a customized optical trapping system from IMPETUX to study the stochastic force dynamics of a model microswimmer (Chlamydomonas reinhardtii).  In particular, they directly measure the stochastic forces generated by the microswimmer using an optical trap via the photon momentum method.

In this work, the authors use optical tweezers to show intracellular softening, fluidification and decrease of active forces in mitosis that is mediated by a surprising role switch between microtubules and actin.

Impetux’s force sensor is used to measure the cytoplasm’s fluidity and sitffness changes of dividing cells.

It is the first time that the cell mechanics is characterized during mitosis from the inside

In this work, researchers used our SENSOCELL  Optical Tweezers platform in combination with Confocal microscopy to study neuron Ca2+ dynamics during axon membrane tether extrusion experiments.

For additional information, have a look at:

In this study, the authors used a customized optical trapping system from IMPETUX to characterize cell membrane elasticity as a new potential biomarker for leukemia cells, comparing measurements for cells treated with anti-cancer drugs and untreated cells. 

Optical trapping has become an optimal choice for biological research at the microscale due to its noninvasiveperformance and accessibility for quantitative studies, especially on the forces involved inbiological processes. However, reliable force measurements depend on the calibration of the opticaltraps, which is different for each experiment and hence requires high control of the local variables,especially of the trapped object geometry. Many biological samples have an elongated, rod-likeshape, such as chromosomes, intracellular organelles (e.g., peroxisomes), membrane tubules, certainmicroalgae, and a wide variety of bacteria and parasites. This type of samples often requires severaloptical traps to stabilize and orient them in the correct spatial direction, making it more difficult todetermine the total force applied. Here, we manipulate glass microcylinders with holographic opticaltweezers and show the accurate measurement of drag forces by calibration-free direct detection ofbeam momentum.

Measuring forces inside living cells is still a challenge due the characteristics of the trapped organelles (non-spherical, unknown size and index of refraction) and the cell cytoplasm surrounding them heterogeneous and dynamic, non-purely viscous). Here, we show how two very recent methods overcome these limitations: on the one hand, forces can be measured in such environment by the direct detection of changes in the light momentum; on the other hand, an active-passive calibration technique provides both the stiffness of the optical trap as well as the local viscoelastic properties of the cell cytoplasm.

 

Papers about IMPETUX’s technology:

 

  • Derek Craig, Alison McDonald, Michael Mazilu, Helen Rendall, Frank Gunn-Moore, and Kishan Dholakia. “ Enhanced Optical Manipulation of Cells Using Antireflection Coated Microparticles”.ACS Photonics, 2 (10), pp 1403–1409, (2015).

    In molecular studies, an optically trapped bead may be functionalized to attach to a specific molecule, whereas in cell studies, direct manipulation with the optical field is usually employed. Using this approach, several methods may be used to measure forces with an optical trap. However, each has its limitations and requires an accurate knowledge of the sample parameters.6,7 In particular, force measurements can be challenging when working with nonspherical particles or in environments with an inhomogeneous viscosity, such as inside the cell. Recent developments in the field are moving toward obtaining direct force measurements by detecting light momentum changes. For this approach, the calibration factor only comes from the detection instrumentation and negates the requirement to recalibrate for changes in experimental conditions”.

  • Xing Ma, Anita Jannasch, Urban-Raphael Albrecht, Kersten Hahn, Albert Miguel-López, Erik Schäffer, and Samuel Sánchez. “Enzyme-Powered Hollow Mesoporous Janus Nanomotors”. Nano Lett., 15 (10), pp 7043–7050, (2015).

    “Using optical tweezers, we directly measured a holding force of 64 ± 16 fN, which was necessary to counteract the effective self-propulsion force generated by a single nanomotor. The successful demonstration of biocompatible enzyme-powered active nanomotors using biologically benign fuels has a great potential for future biomedical applications.”

 In this work, the authors show the feasibility of combining optical tweezers (single-beam gradient traps) with the determination of forces using the measurement of the light momentum change.

Impetux

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