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News

CONFERENCES: Nanoengineering for Mechanobiology, Nov 30- Dec 3, 2020

IMPETUX will be present at the Nanoengineering for Mechanobiology symposium, hold on November 30- December 3, 2020  on-line.

We will be participating with an interesting talk on Monday the 30th of November:

 SENSOCELL: a novel optical tweezers concept for cell and tissue mechanobiology

There we will be answering all your questions during the Q&A scheduled Zoom sessions and at our virtual booth.

 If you are interested in our latest developments an applications of SENSOCELL, and how our distinctive technology can boost your research, do not hesitate to join us!

read more…

The characterization of cell membrane elasticity as a new potential biomarker for leukemia cells with IMPETUX technology

Researchers from the Optics Research Unit at De La Salle University and AIST-Osaka University have recently published and study where they have used a customized optical trapping system from IMPETUX to characterize cell membrane elasticity as a new potential biomarker for leukemia cells, comparing measurements for cells treated with anti-cancer drugs and untreated cells.

You can  review all the  results here :

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Cytoskeleton Day, 10 November 2020, Paris

IMPETUX will be present at the Cytoskeleton Day,  hold on November  10, 2020  in Paris.

We will be sharing interesting data acquired with SENSOCELL, the unique optical tweezers platform for mechanobiology, with a short talk at 11:15 h

If you are interested in our latest developments an applications of SENSOCELL, do not hesitate to join us!

WherePARIS CYTOSKELETON DAY: A meet held entirely online

When → Tuesday, November 10, 2020, at 11:15h

How → Register here:

Measuring forces, deformation and mechanical properties of cell nucleus with SENSOCELL

Researchers from Insitut de Ciències Fotòniques (ICFO) , Centre for Genomic Regulation (CRG), Universitat Pompeu Fabra (UPF) and University of Applied Science Upper Austria have recently published an amazing Science article in where they show that the nucleus, the biggest organelle in the cell, translates cell shape changes into a deformation signal regulating cell behavior.

In this study, our optical tweezers system SENSOCELL has been used to precisely measure the forces applied to the nucleus, determine its deformation and obtain its mechanical properties.

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WEBINAR OCTOBER 22, 2020

Introducing SENSOCELL: Optical Tweezers for Mechanobiology

DATE: October 22, 2020
Time: 19h CET (Paris, GMT+2)

Optical tweezers for mechanobiology SENSOCELL

SENSOCELL is an optical tweezers system, designed for mechanobiology studies in living cells and 3D tissues. It allows manipulating and deforming cells as whole or even endogenous structures such as the cell membrane, the cell nucleus, vesicles and other organelles while tracking the in vivo biological forces involved thanks to its unique direct force sensor technology.

read more…

Events

CONFERENCES: Nanoengineering for Mechanobiology, Nov 30- Dec 3, 2020

IMPETUX will be present at the Nanoengineering for Mechanobiology symposium, hold on November 30- December 3, 2020  on-line.

We will be participating with an interesting talk on Monday the 30th of November:

 SENSOCELL: a novel optical tweezers concept for cell and tissue mechanobiology

There we will be answering all your questions during the Q&A scheduled Zoom sessions and at our virtual booth.

 If you are interested in our latest developments an applications of SENSOCELL, and how our distinctive technology can boost your research, do not hesitate to join us!

read more…

Cytoskeleton Day, 10 November 2020, Paris

IMPETUX will be present at the Cytoskeleton Day,  hold on November  10, 2020  in Paris.

We will be sharing interesting data acquired with SENSOCELL, the unique optical tweezers platform for mechanobiology, with a short talk at 11:15 h

If you are interested in our latest developments an applications of SENSOCELL, do not hesitate to join us!

WherePARIS CYTOSKELETON DAY: A meet held entirely online

When → Tuesday, November 10, 2020, at 11:15h

How → Register here:

Scientific Publications

Here you will find useful material published related with our technology and products.

 

Papers:

 

For additional information, have a look at:

In this study, the authors used a customized optical trapping system from IMPETUX to characterize cell membrane elasticity as a new potential biomarker for leukemia cells, comparing measurements for cells treated with anti-cancer drugs and untreated cells.

 

Optical trapping has become an optimal choice for biological research at the microscale due to its noninvasiveperformance and accessibility for quantitative studies, especially on the forces involved inbiological processes. However, reliable force measurements depend on the calibration of the opticaltraps, which is different for each experiment and hence requires high control of the local variables,especially of the trapped object geometry. Many biological samples have an elongated, rod-likeshape, such as chromosomes, intracellular organelles (e.g., peroxisomes), membrane tubules, certainmicroalgae, and a wide variety of bacteria and parasites. This type of samples often requires severaloptical traps to stabilize and orient them in the correct spatial direction, making it more difficult todetermine the total force applied. Here, we manipulate glass microcylinders with holographic opticaltweezers and show the accurate measurement of drag forces by calibration-free direct detection ofbeam momentum.

Measuring forces inside living cells is still a challenge due the characteristics of the trapped organelles (non-spherical, unknown size and index of refraction) and the cell cytoplasm surrounding them heterogeneous and dynamic, non-purely viscous). Here, we show how two very recent methods overcome these limitations: on the one hand, forces can be measured in such environment by the direct detection of changes in the light momentum; on the other hand, an active-passive calibration technique provides both the stiffness of the optical trap as well as the local viscoelastic properties of the cell cytoplasm.

  • Martín-Badosa, F. Català, J. Mas, M. Montes-Usategui, A. Farré, F. Marsà. “Force measurement in the manipulation of complex samples with holographic optical tweezers” 15th workshop on Information Optics (WIO), 2016.
  • Derek Craig, Alison McDonald, Michael Mazilu, Helen Rendall, Frank Gunn-Moore, and Kishan Dholakia. “ Enhanced Optical Manipulation of Cells Using Antireflection Coated Microparticles”.ACS Photonics, 2 (10), pp 1403–1409, (2015).

    In molecular studies, an optically trapped bead may be functionalized to attach to a specific molecule, whereas in cell studies, direct manipulation with the optical field is usually employed. Using this approach, several methods may be used to measure forces with an optical trap. However, each has its limitations and requires an accurate knowledge of the sample parameters.6,7 In particular, force measurements can be challenging when working with nonspherical particles or in environments with an inhomogeneous viscosity, such as inside the cell. Recent developments in the field are moving toward obtaining direct force measurements by detecting light momentum changes. For this approach, the calibration factor only comes from the detection instrumentation and negates the requirement to recalibrate for changes in experimental conditions”.

  • Xing Ma, Anita Jannasch, Urban-Raphael Albrecht, Kersten Hahn, Albert Miguel-López, Erik Schäffer, and Samuel Sánchez. “Enzyme-Powered Hollow Mesoporous Janus Nanomotors”. Nano Lett., 15 (10), pp 7043–7050, (2015).

    “Using optical tweezers, we directly measured a holding force of 64 ± 16 fN, which was necessary to counteract the effective self-propulsion force generated by a single nanomotor. The successful demonstration of biocompatible enzyme-powered active nanomotors using biologically benign fuels has a great potential for future biomedical applications.”

 In this work, the authors show the feasibility of combining optical tweezers (single-beam gradient traps) with the determination of forces using the measurement of the light momentum change.

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