Customer Stories


To deep more into what the IMPETUX technology users are achieving, here we share a list of their latest publications:



September 2023

The laminin-keratin link shields the nucleus from mechanical deformation and signaling

Zanetta Kechagia, Pablo Sáez, Manuel Gómez-González, Martín Zamarbide, Ion Andreu, Thijs Koorman, Amy E.M. Beedle, Patrick W.B. Derksen, Xavier Trepat, Marino Arroyo,  Pere Roca-Cusachs

Nature Materials:

In this work, the labs of Pere Roca-Cusachs and Xavier Trepat (IBEC, Barcelona) investigate how the rigidity of a laminin based extracellular environment influence mechanotransduction in mammary epithelial cell models.

You will find the article here.


January 2023

Persistent confined migration confers permanent nuclear and functional changes in migrating cells.

Ana de Lope-Planelles, Raquel González-Novo, Elena Madrazo,  Héctor Zamora-Carreras, Verónica Torrano, Horacio López-Menéndez, Pedro Roda-Navarro,  Francisco Monroy, Javier Redondo-Muñoz



  • Persistent confined migration promotes permanent mophological changes.

  • Lamin B1 is redistribution in the nucleus of migratory altered cells.

  • Migratory-altered cells exhibit transcriptional and functional changes related to cell migration and survival.

  • Actin polymerization controls nuclear changes induced by cell migration.

This article is a pre-print, and you will find it here



July  2022

A rigidity phase transition of Stomatin condensates governs a switch from transport to mechanotrasnduction

Neus Sanfeliu-Cerdán, Borja Mateos, Carla Garcia-Cabau, Frederic Català-Castro, Maria Ribera, Iris Ruider, Montserrat Porta-de-la-Riva, Stefan Wieser, Xavier Salvatella,  Michael Krieg


This article is a pre-print, and you will find it here


May 2022

Osanger regression characterizes living systems in passive measurements

Till M. Muenker,  Gabriel Knotz,  Matthias Krüger,  Timo Betz


This article is a pre-print, and you will find it here


March  2022

Video Protocol for Direct Force Measurements of Subcellular Mechanics in Confinement using Optical Tweezers

Frederic Català-Castro, Valeria Venturini, Santiago Ortiz-Vásquez, Verena Ruprecht, Michael Krieg

JoVE Journal: DOI: 10.3791/62865-v

Here, the authors present a protocol to investigate the intracellular mechanical properties of isolated embryonic zebrafish cells in three-dimensional confinement with direct force measurement by an optical trap.

If interested in intracellular and nuclear mechanics,  have to look at it here.



November 2021

Non-equilibrium fluctuations and nonlinear response of an active bath

 Hunter Seyforth, Mauricio Gomez, W. Benjamin Rogers, Jennifer L. Ross, Wylie W. Ahmed

In this work, Dr Ahmed ( leader of  the Laboratory for Soft, Living, and Active Matter)  et al.,  analyze the dynamics of a passive colloidal probe immersed in an active bath using Optical tweezers in combination with our force spectroscopy technology .

If you are interested in soft condensed matter and nonlinear rheology have a look at it.

This article is a pre-print, and you will find it at: arXiv:2110.15917 [cond-mat.soft]


October 2021

Intracellular softening and increased viscoelastic fluidity during division

Sebastian Hurst, Bart E. Vos, Matthias Brandt & Timo Betz

Nature Physics (2021).

In this work, the authors use optical tweezers to show intracellular softening, fluidification and decrease of active forces in mitosis that is mediated by a surprising role switch between microtubules and actin.

Impetux's force sensor is used to measure the cytoplasm's fluidity and stiffness changes of dividing cells.

It is the first time that the cell mechanics is characterized during mitosis from the inside



September 2021

An asymmetric mechanical code ciphers curvature-dependent proprioceptor activity

Ravi Das, Li-Chun Lin, Frederic Català-Castro, Nawaphat Malaiwong, Neus Sanfeliu,  Montserrat Porta-de-la-Riva, Aleksandra Pidde, Michael Krieg.

SCIENCE ADVANCES 17 Sep 2021 Vol 7, Issue 38 DOI: 10.1126/sciadv.abg4617

In this work, the mechanical activation of neuronal activity in C. elegans is studied by means of genome editing, modelling and direct force spectroscopy integrated in SENSOCELL optical tweezersThe system is also combined with spinning-disk confocal microscopy to monitor Ca2+ ion channel dynamics during axon membrane tether extrusion experiments.



August 2021

Direct Force Measurements of Subcellular Mechanics in Confinement using Optical Tweezers - JOVE protocol.

Frederic Català-Castro, Valeria Venturini, Santiago Ortiz-Vásquez, Verena Ruprecht, Michael Krieg.

J. Vis. Exp.(174), e62865, doi:10.3791/62865 (2021).

In this article, the authors describe in detail a new method and protocol using SENSOCELL optical tweezers to measure the forces and material properties that shape the cell nucleus inside living cells, exemplified on adherent cells and mechanically confined cells.

According to the authors,

The presented method is straightforward and can easily be extended to investigate the mechanics of other subcellular compartments, e.g., mitochondria, stress-fibers, and endosomes.

To deepen into the details of this protocol and look at the representative results shown, you can read the full article here.

For additional information, have a look at:

Our Customer Story.

Our Nucleus Mechanics Application page.



July 2021

The force loading rate drives cell mechanosensing through both reinforcement and fluidization

Ion Andreu, Bryan Falcones, Sebastian Hurst, Nimesh Chahare, Xarxa Quiroga, Anabel-Lise Le Roux, Zanetta Kechagia, Amy E.M. Beedle, Alberto Elósegui-Artola, Xavier Trepat, Ramon Farré, Timo Betz, Isaac Almendros, Pere Roca-Cusachs

         Nature Communications 12 | 4229 (2021) |  

This work provides a unifying mechanism to understand how cells respond not only to directly applied forces, but also to passive mechanical stimuli such as tissue rigidity or ECM ligand distribution.

To know the details of this interesting work have a look at the article here.

You can also read an interesting highlight by Jessica L. Teo here



March 2021

Stochastic force dynamics of the model microswimmer Chlamydomonas reinhardtii: Active forces and energetics

Corbyn Jones, Mauricio Gomez, Ryan M. Muoio, Alex Vidal, Anthony Mcknight, Nicholas D. Brubaker, Wylie W. Ahmed.

Phys. Rev. E 103, 032403 – Published 5 March 2021.

DOI: 10.1103/PhysRevE.103.032403

In this work, the authors use a customized optical trapping system from IMPETUX to study the stochastic force dynamics of a model microswimmer algaes (Chlamydomonas reinhardtii).  In particular, they directly measure the stochastic forces generated by the algaes using an optical trap via the photon momentum method.

The authors of this article have been the recipients of the 2023 Irwin Oppenheim Award for best paper by early career scientists published in Physical Review E.

Their article was selected for elucidating the stochastic force dynamics of a model biological microswimmer using an innovative combination of direct model-independent force measurement, simulation, and analytical modeling.

To know the details of this interesting work have a look at the article here. 


December 2020

In-vitro study of monocytic THP-1 leukemia cell membrane elasticity with a single-cell microfluidic-assisted optical trapping system

R. Ombid, G. Oyong, E. Cabrera, W. Espulgar, M. Saito, E. Tamiya, and R. Pobre

Biomed. Opt. Express 11, 6027-6037 (2020).

In this study, the authors used a customized optical trapping system from IMPETUX to characterize cell membrane elasticity as a new potential biomarker for leukemia cells, comparing measurements for cells treated with anti-cancer drugs and untreated cells.



October 2020

The nucleus measures shape changes for cellular proprioception to control dynamic cell behaviour

V. Venturi, F. Pezzano, F. Català-Castro, H.- M. Häkkinen, S. Jiménez-Delgado, M. Colomer-Rosell, M. Marro-Sánchez, Q. Tolosa-Ramon, S. Paz-López, M. A. Valverde, P. Loza-Alvarez, M. Krieg, S. Wieser and V. Ruprecht

SCIENCE | 16 Oct 2020: Vol. 370, Issue 6514, eaba2644. DOI: 10.1126/science.aba2644

For additional information, have a look at:

Our Customer Story.
CRG’s web page
ICFO’S web page

SCB AWARD finalist  for a scientific publication considered to have contributed most significantly to the advance of biological science field, carried out mainly in Catalan-speaking territories


May 2018

Optical Force Sensing with Cylindrical Microcontainers

R. Meissner, N. Oliver and C.Denz

Part. Part. Syst. Charact. 2018, 1800062.



November 2017

Influence of experimental parameters on the laser heating of an optical trap 

F.Català, F. Marsà, M. Montes Usategui, A. Farré & E. Martín-Badosa

Sci. Rep. 7, 16052; doi:10.1038/s41598-017-15904-6 (2017).


Fabruary 2017

Extending calibration-free force measurements to optically-trapped rod-shaped samples

Frederic Català, Ferran Marsà, Mario Montes-Usategui, Arnau Farré and Estela Martín-Badosa 

Sci. Rep. 7, 42960; doi: 10.1038/srep42960 (2017).



July 2016

Optical tweezers for force measurements and rheological studies on biological samples

R. Bola, F. Català. M. Montes-Usategui, E. Martín-Badosa.

15th workshop on Information Optics (WIO), 2016.