How SENSOCELL offers the possibility to address fundamental questions about the mechanobiology of cells
SENSOCELL is the ideal tool to bring mechanobiology and force measurements into animal model systems, such as C. elegans, Drosophila or Zebrafish.Dr. Michael Krieg leads the Neurophotonics and Mechanical Systems Biology group at the Institute of Photonics Sciences...
The study of subcellular mechanics is essential to understand how, among other biological processes, cells proliferate, migrate or differentiate. Indeed, cells, decode mechanical signals via the presence of force-sensitive subcellular and molecular elements that...
Understanding how cells respond to directly applied forces and to passive mechanical stimuli with SENSOCELL force spectroscopy technology
It is known that physical forces regulate cell response and determine essential processes. In the publication : The force loading rate drives cell mechanosensing through both reinforcement and fluidization Ion Andreu, Bryan Falcones, Sebastian Hurst, Nimesh Chahare,...
To deep more into what the IMPETUX technology users are achieving, here we share a list of their latest publications:
Direct Force Measurements of Subcellular Mechanics in Confinement using Optical Tweezers - JOVE protocol.
Frederic Català-Castro, Valeria Venturini, Santiago Ortiz-Vásquez, Verena Ruprecht, Michael Krieg.
In this article, the authors describe in detail a new method and protocol using SENSOCELL optical tweezers to measure the forces and material properties that shape the cell nucleus inside living cells, exemplified on adherent cells and mechanically confined cells. According to the authors, the presented method is straightforward and can easily be extended to investigate the mechanics of other subcellular compartments, e.g., mitochondria, stress-fibers, and endosomes.
To deepen into the details of this protocol and look at the representative results shown, you can read the full article here.
For additional information, have a look at:
Our Customer Story.
Ion Andreu, Bryan Falcones, Sebastian Hurst, Nimesh Chahare, Xarxa Quiroga, Anabel-Lise Le Roux, Zanetta Kechagia, Amy E.M. Beedle, Alberto Elósegui-Artola, Xavier Trepat, Ramon Farré, Timo Betz, Isaac Almendros, Pere Roca-Cusachs
This work provides a unifying mechanism to understand how cells respond not only to directly applied forces, but also to passive mechanical stimuli such as tissue rigidity or ECM ligand distribution.
To know the details of this interesting work have a look at the article here.
You can also read an interesting highlight by Jessica L. Teo here
Stochastic force dynamics of the model microswimmer Chlamydomonas reinhardtii: Active forces and energetics
Corbyn Jones, Mauricio Gomez, Ryan M. Muoio, Alex Vidal, Anthony Mcknight, Nicholas D. Brubaker, Wylie W. Ahmed.
In this work, the authors use a customized optical trapping system from IMPETUX to study the stochastic force dynamics of a model microswimmer algaes (Chlamydomonas reinhardtii). In particular, they directly measure the stochastic forces generated by the algaes using an optical trap via the photon momentum method.
Intracellular softening and fluidification reveals a mechanical switch of cytoskeletal material contributions during division
Sebastian Hurst, Bart E. Vos, Timo Betz
This article is a preprint
In this work, the authors use optical tweezers to show intracellular softening, fluidification and decrease of active forces in mitosis that is mediated by a surprising role switch between microtubules and actin.
Impetux's force sensor is used to measure the cytoplasm's fluidity and stiffness changes of dividing cells.
It is the first time that the cell mechanics is characterized during mitosis from the inside
Mechanical Stretch Inhibition Sensitizes Proprioceptors to Compressive Stresses
Ravi Das, Li-Chun Lin, Frederic Català-Castro, Nawaphat Malaiwong, Neus Sanfeliu, Montserrat Porta-de-la-Riva, Aleksandra Pidde, Michael Krieg.
This article is a preprint.
In this work, researchers used our SENSOCELL Optical Tweezers platform in combination with Confocal microscopy to study neuron Ca2+ dynamics during axon membrane tether extrusion experiments.
The nucleus measures shape changes for cellular proprioception to control dynamic cell behaviour
V. Venturi, F. Pezzano, F. Català-Castro, H.- M. Häkkinen, S. Jiménez-Delgado, M. Colomer-Rosell, M. Marro-Sánchez, Q. Tolosa-Ramon, S. Paz-López, M. A. Valverde, P. Loza-Alvarez, M. Krieg, S. Wieser and V. Ruprecht
For additional information, have a look at:
SCB AWARD finalist for a scientific publication considered to have contributed most significantly to the advance of biological science field, carried out mainly in Catalan-speaking territories
In-vitro study of monocytic THP-1 leukemia cell membrane elasticity with a single-cell microfluidic-assisted optical trapping system
R. Ombid, G. Oyong, E. Cabrera, W. Espulgar, M. Saito, E. Tamiya, and R. Pobre
In this study, the authors used a customized optical trapping system from IMPETUX to characterize cell membrane elasticity as a new potential biomarker for leukemia cells, comparing measurements for cells treated with anti-cancer drugs and untreated cells.
Optical Force Sensing with Cylindrical Microcontainers
R. Meissner, N. Oliver and C.Denz
F.Català, F. Marsà, M. Montes Usategui, A. Farré & E. Martín-Badosa
Frederic Català, Ferran Marsà, Mario Montes-Usategui, Arnau Farré and Estela Martín-Badosa
Optical tweezers for force measurements and rheological studies on biological samples
R. Bola, F. Català. M. Montes-Usategui, E. Martín-Badosa.
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