Customer Stories

To deep more into what the IMPETUX technology users are achieving, here we share a list of their latest publications:

 

 

August 2021


Direct Force Measurements of Subcellular Mechanics in Confinement using Optical Tweezers - JOVE protocol.

Frederic Català-Castro, Valeria Venturini, Santiago Ortiz-Vásquez, Verena Ruprecht, Michael Krieg.

J. Vis. Exp.(174), e62865, doi:10.3791/62865 (2021).

In this article, the authors describe in detail a new method and protocol using SENSOCELL optical tweezers to measure the forces and material properties that shape the cell nucleus inside living cells, exemplified on adherent cells and mechanically confined cells. According to the authors, the presented method is straightforward and can easily be extended to investigate the mechanics of other subcellular compartments, e.g., mitochondria, stress-fibers, and endosomes.

To deepen into the details of this protocol and look at the representative results shown, you can read the full article here.

For additional information, have a look at:

Our Customer Story.

Our Nucleus Mechanics Application page.

 

 

July 2021


The force loading rate drives cell mechanosensing through both reinforcement and fluidization

Ion Andreu, Bryan Falcones, Sebastian Hurst, Nimesh Chahare, Xarxa Quiroga, Anabel-Lise Le Roux, Zanetta Kechagia, Amy E.M. Beedle, Alberto Elósegui-Artola, Xavier Trepat, Ramon Farré, Timo Betz, Isaac Almendros, Pere Roca-Cusachs

          Nature Communications 12 | 4229 (2021) | https://doi.org/10.1038/s41467-021-24383-3

This work provides a unifying mechanism to understand how cells respond not only to directly applied forces, but also to passive mechanical stimuli such as tissue rigidity or ECM ligand distribution.

To know the details of this interesting work have a look at the article here.

You can also read an interesting highlight by Jessica L. Teo here

 

 

March 2021


Stochastic force dynamics of the model microswimmer Chlamydomonas reinhardtii: Active forces and energetics

Corbyn Jones, Mauricio Gomez, Ryan M. Muoio, Alex Vidal, Anthony Mcknight, Nicholas D. Brubaker, Wylie W. Ahmed.

Phys. Rev. E 103, 032403 – Published 5 March 2021.

DOI: 10.1103/PhysRevE.103.032403

In this work, the authors use a customized optical trapping system from IMPETUX to study the stochastic force dynamics of a model microswimmer algaes (Chlamydomonas reinhardtii).  In particular, they directly measure the stochastic forces generated by the algaes using an optical trap via the photon momentum method.

 

 

January 2021


Intracellular softening and fluidification reveals a mechanical switch of cytoskeletal material contributions during division

Sebastian Hurst, Bart E. Vos, Timo Betz

BioRxiv 2021.01.07.425761;

doi: https://doi.org/10.1101/2021.01.07.425761.

This article is a preprint

In this work, the authors use optical tweezers to show intracellular softening, fluidification and decrease of active forces in mitosis that is mediated by a surprising role switch between microtubules and actin.

Impetux's force sensor is used to measure the cytoplasm's fluidity and stiffness changes of dividing cells.

It is the first time that the cell mechanics is characterized during mitosis from the inside

 

 

January 2021


Mechanical Stretch Inhibition Sensitizes Proprioceptors to Compressive Stresses

Ravi Das, Li-Chun Lin, Frederic Català-Castro, Nawaphat Malaiwong, Neus Sanfeliu,  Montserrat Porta-de-la-Riva, Aleksandra Pidde, Michael Krieg.

BioRxiv 2020.12.30.422571; doi: https://doi.org/10.1101/2020.12.30.422571.

This article is a preprint.

In this work, researchers used our SENSOCELL Optical Tweezers platform in combination with Confocal microscopy to study neuron Ca2+ dynamics during axon membrane tether extrusion experiments.

 

 

October 2020


The nucleus measures shape changes for cellular proprioception to control dynamic cell behaviour

V. Venturi, F. Pezzano, F. Català-Castro, H.- M. Häkkinen, S. Jiménez-Delgado, M. Colomer-Rosell, M. Marro-Sánchez, Q. Tolosa-Ramon, S. Paz-López, M. A. Valverde, P. Loza-Alvarez, M. Krieg, S. Wieser and V. Ruprecht

SCIENCE | 16 Oct 2020: Vol. 370, Issue 6514, eaba2644. DOI: 10.1126/science.aba2644

For additional information, have a look at:

Our Customer Story.
CRG’s web page
ICFO’S web page

SCB AWARD finalist  for a scientific publication considered to have contributed most significantly to the advance of biological science field, carried out mainly in Catalan-speaking territories

 

December 2020


In-vitro study of monocytic THP-1 leukemia cell membrane elasticity with a single-cell microfluidic-assisted optical trapping system

R. Ombid, G. Oyong, E. Cabrera, W. Espulgar, M. Saito, E. Tamiya, and R. Pobre

Biomed. Opt. Express 11, 6027-6037 (2020).

In this study, the authors used a customized optical trapping system from IMPETUX to characterize cell membrane elasticity as a new potential biomarker for leukemia cells, comparing measurements for cells treated with anti-cancer drugs and untreated cells.

 

 

May 2018


Optical Force Sensing with Cylindrical Microcontainers

R. Meissner, N. Oliver and C.Denz

Part. Part. Syst. Charact. 2018, 1800062.

 

 

November 2017


Influence of experimental parameters on the laser heating of an optical trap 

F.Català, F. Marsà, M. Montes Usategui, A. Farré & E. Martín-Badosa

Sci. Rep. 7, 16052; doi:10.1038/s41598-017-15904-6 (2017).

 

Fabruary 2017


Extending calibration-free force measurements to optically-trapped rod-shaped samples

Frederic Català, Ferran Marsà, Mario Montes-Usategui, Arnau Farré and Estela Martín-Badosa 

Sci. Rep. 7, 42960; doi: 10.1038/srep42960 (2017).

 

 

July 2016


Optical tweezers for force measurements and rheological studies on biological samples

R. Bola, F. Català. M. Montes-Usategui, E. Martín-Badosa.

15th workshop on Information Optics (WIO), 2016.

 

Impetux